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Continue on foot northward on Berliner Stra�e, past the green building (INF 580) and taking the paved footpath on the left to INF 581 (pink building).

On Foot: The Division of Biostatistics is located approximately 2 km from the main train station and can be reached on foot in about 20 minutes.

Exit the main station by the side door (straight ahead coming from the tracks), cross the Kurf�rstenanlage (large 4-lane-street) and head north.

Continue across the intersection of Berliner Stra�e / Im Neuenheimer Feld, passing INF 580 (green building), until you reach the paved footpath on the left, which leads to INF 581 (pink building).

Inside Building 581 (also known as TP4): Enter via the glass doors at the main entrance and turn left toward the elevators.

On Foot: The Division of Biostatistics is located approximately 2 km from the main train station and can be reached on foot in about 20 minutes.

Exit the main station by the side door (straight ahead coming from the tracks), cross the Kurf�rstenanlage (large 4-lane-street) and head north.

Continue across the intersection of Berliner Stra�e / Im Neuenheimer Feld, passing INF 580 (green building), until you reach the paved footpath on the left, which leads to INF 581 (pink building).

Inside Building 581 (also known as TP4): Enter via the glass doors at the main entrance and turn left toward the elevators.

The chromatin remodelling complex NoRC safeguards genome stability by heterochromatin formation at telomeres and centromeres

As loss of telomeric heterochromatin correlates with telomere elongation [11, 12], depletion of NoRC should lead to changes in telomere homeostasis.

As the interaction with PML-NBs indicates increased telomeric recombination, we assayed the frequency of telomeric sister chromatid exchange on metaphase spreads of control and TIP5-deficient cells by CO-FISH (chromosome orientation-FISH) using differently labelled strand-specific telomeric probes.

Moreover, multiplex-FISH (M-FISH) on metaphase spreads showed a >2-fold increase in telomere translocations as early as 32 h after TIP5 knockdown, emphasizing the impact of NoRC on telomere and chromosome stability (supplementary Table S2 online).

However, while the former study has been performed with a TIP5-deficient cell line that exhibited pronounced alterations in morphology and might have been transformed during long-term cultivation, we have now monitored functional consequences of TIP5 depletion early (60 h) after transfection and at the single-cell level.

Although the molecular details underlying NoRC function at telomeres and centromeres need further analysis, we propose that NoRC, surely among other factors as well, triggers the establishment or maintenance of compact chromatin structure at other genomic sequences with wide-ranging implications for genome structure and function.